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and measure the absorbance (OD value) of each well sequentially at a wavelength of 450nm
immunoglobulin class conversion to IgE
and HRP conjugate are sequentially added to microwells pre-coated with a collagen type II (COL2) capture antibody
serving as the template for transcription
The intensity of the color is positively correlated with the amount of Interleukin 31 (IL31) in the sample
Mouse SP7 ELISA Kit Size:96T and measure the absorbance (ODProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and